Rat Hybridoma Development
Two female Sprague Dawley rats will be immunized and rat-mouse hetero or rat-rat hybridoma will be developed through fusion of rat splenocytes or lymph node cells with myeloma cells. A proprietary protocol will be used to reduce half the processing and generate superior-quality, custom hybridomas within 2 months’ time or less.
With our Clone Express Immunization protocol, you can have hybridomas in 2 months or will have antibody guaranteed work for your applications.
We provide specific packages designed to screen antibodies for your intended use, including:
-> Ab ELISA package
-> Ab Western package
-> Ab Histo package—specially designed to screen antibodies for IHC applications
Stepwise Protocol for Monoclonal Antibody Development - Express
|Cat#||Description||Materials and Information Request||Product Delivery||Time frame|
|Phase 0 (optional)||CY0001||Immunogen Preparation and conjugation||Name and intended use||Immunogen and screening materials for immunization||4-6 weeks|
|Phase 1||CY0002||Immunization and serum screening||Immunogen1 Screening materials2||ELISA titer report||2 weeks for express protocol3|
|Phase 2||CY0003||Cell fusion, screening, and subcloning||Confirmation on selected clones||Screening and subcloning report||3 weeks|
|Phase 3 (optional)||CY0004||monoclonal antibody production.||Isotyping report; 3-5 final clones, up to 10 mg antibodies||4 weeks|
1. Peptide 5 mg (need to be conjugated to KLH/BSA) or protein 2 mg, or other forms of immunogen
2. Usually immunogen in different forms
3. Immunization of drained multiple lymph notes, and fusion will be done by taking the notes.