Rat Hybridoma Development

Two female Sprague Dawley rats will be immunized and rat-mouse hetero or rat-rat hybridoma will be developed by fusion of rat splenocytes or lymph node cells with myeloma cells. A proprietary protocol will be used to reduce the processing time by 50% and generates superior quality custom hybridomas in 2 months or less.

With our Clone Express Immunization protocol, you can have hybridomas in 2 months or will have antibody guaranteed work for your applications.

Turn around Time

With our CEI methodology we can provide you with specific Antibodies within 2 months timeframe.

Monoclonal Antibody Development & Screening Packages

We provide 3 packages specifically designed to screen antibodies for your intended use.

Stepwise Protocol for Monoclonal Antibody Development

	Cat#	Description	Information Request	Product Delivery	Time frame
Pre phase (optional)		Peptide synthesis and conjugation to KLH and BSA	Name and intended use of the protein of interest	1.5 mg of peptide-KLH for immunization; 2. 5 mg of peptide-BSA for screening	4-6 weeks
Phase 1		Immunization and serum screening	Immunogen 1Screening materials2	ELISA titer report	2 weeks for express protocol Clone Express3
Phase 2		Cell fusion and parental clone screening		Screening report	3 weeks
Phase 3		Subcloning on selected positive parental clones	Confirmation on selected clones	Subcloning report	2 weeks
Phase 4 (optional)		Monoclonal antibody production		Isotyping report; 3-5 final clones, up to 10 mg antibodies	4 weeks

1. Peptide 5 mg (need to be conjugated to KLH/BSA) or protein 2 mg, or other forms of immunogen

2. Usually immunogen in different forms

3. Immunization of drained multiple lymph notes, and fusion will be done by taking the notes.