Purification:

Cyto360 purifies monoclonal and polyclonal antibodies utilizing a wide array of protein purification methods for production quantities (grams) as well as research quantities (milligrams). We are happy to discuss clients’ purification requirements to find a method to best fit each client’s needs.
Our purification packages include:

Protein G affinity chromatography

Protein A affinity chromatography

Melon Gel IgG purification

Mouse IgM purification

Ammonium sulfate precipitation

 

Antibody purification by Protein A/G.

Cyto360’s protein A/G resins are effective for affinity purification of IgG from serum and other fluids of many species. Protein A/G is especially suited for polyclonal antibody purification because it binds the broadest range of antibody species and subclasses. Protein A/G also binds well to all mouse IgG subclasses but does not bind mouse IgA or IgM.

 

Antibody purification by Melon Gel

Our Melon Gel IgG purification method provides extremely fast, easy and mild purification of antibodies from serum and other sample types. Antibodies from many species are recovered with greater than 90% yield and greater than 80% purity. It is especially useful for rat IgG purification.

 

Mouse IgM purification

IgM binds poorly to protein A and protein G, making one-step IgM purification difficult. We use an immobilized mannan binding protein (MBP) support, which is a highly effective method for mouse IgM purification.