Mouse Hybridoma Development - Express

Cyto360 uses a Clone Express Immunization (CEI) protocol to save half the processing time and generate superior-quality, custom hybridomas for your experimental needs within 2 months’ time or less.

DEVELOPMENT PACKAGES:

We provide specific packages designed to screen antibodies for your intended use, including:
-> Ab ELISA package
-> Ab Western package
-> Ab Histo package—specially designed to screen antibodies for IHC applications

Stepwise Protocol for Monoclonal Antibody Development

  Cat# Description Materials and Information Request Product Delivery Time frame
Phase 0 (optional) CY0001 Immunogen Preparation and conjugation Name and intended use Immunogen and screening materials for immunization 4-6 weeks
Phase 1 CY0002 Immunization and serum screening Immunogen1 Screening materials2 ELISA titer report 3 weeks for express protocol3
Phase 2 CY0003 Cell fusion, screening, and subcloning Confirmation on selected clones Screening and subcloning report 3 weeks
Phase 3 (optional) CY0004 monoclonal antibody production.   Isotyping report; 3-5 final clones, up to 10 mg antibodies 4 weeks


1. Peptide 5 mg (need to be conjugated to KLH/BSA) or protein 2 mg, or other forms of immunogen
2. Usually immunogen in different forms
3. Immunization of drained multiple lymph notes, and fusion will be done by taking the notes.