Mouse Hybridoma Development - Express
Cyto360 uses a Clone Express Immunization (CEI) protocol to save half the processing time and generate superior-quality, custom hybridomas for your experimental needs within 2 months’ time or less.
We provide specific packages designed to screen antibodies for your intended use, including:
-> Ab ELISA package
-> Ab Western package
-> Ab Histo package—specially designed to screen antibodies for IHC applications
Stepwise Protocol for Monoclonal Antibody Development
|Cat#||Description||Materials and Information Request||Product Delivery||Time frame|
|Phase 0 (optional)||CY0001||Immunogen Preparation and conjugation||Name and intended use||Immunogen and screening materials for immunization||4-6 weeks|
|Phase 1||CY0002||Immunization and serum screening||Immunogen1 Screening materials2||ELISA titer report||3 weeks for express protocol3|
|Phase 2||CY0003||Cell fusion, screening, and subcloning||Confirmation on selected clones||Screening and subcloning report||3 weeks|
|Phase 3 (optional)||CY0004||monoclonal antibody production.||Isotyping report; 3-5 final clones, up to 10 mg antibodies||4 weeks|
1. Peptide 5 mg (need to be conjugated to KLH/BSA) or protein 2 mg, or other forms of immunogen
2. Usually immunogen in different forms
3. Immunization of drained multiple lymph notes, and fusion will be done by taking the notes.